Coding

Part:BBa_K1998005:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-12)


psbCA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1043


Design Notes

Genes were codon optimised for bacteria using optimiser tool Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.



Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References